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Image Search Results
Journal: The Journal of Experimental Medicine
Article Title: Expression Levels of B Cell Surface Immunoglobulin Regulate Efficiency of Allelic Exclusion and Size of Autoreactive B-1 Cell Compartment
doi:
Figure Lengend Snippet: Tg H chain expression in B cells of heterozygous or homozygous H+L and H3 Tg mice. Cells were isolated from bone marrow (A) and spleen (B) of indicated mice at 10–14 wk of age and stained with FITC-conjugated antiallotypic antibody for IgM a (Tg) and PE-conjugated antiallotypic antibody for IgM b (endogenous). Control stainings of IgM a (BALB/c) and IgM b (C57BL/6) spleen cells are shown. The percentages of cells of each gated region in total viable cells are indicated. Total numbers of the cells with endogenous H chains in heterozygous or homozygous H+L5, H+L6, and H3 Tg mice are shown (C). Spleen cells were stained and analyzed from at least three mice of each Tg line. Numbers of indicated cells were calculated by (percentage of indicated cells in viable cells) × (No. of viable cells). Results are expressed as mean ± SD.
Article Snippet: FITC- or Cy-Chrome ® –conjugated anti–mouse CD45R (B220), FITC- or PE-conjugated anti–mouse IgM a (Igh-6 a ), FITC- or PE-conjugated
Techniques: Expressing, Isolation, Staining
Journal: The Journal of Experimental Medicine
Article Title: Expression Levels of B Cell Surface Immunoglobulin Regulate Efficiency of Allelic Exclusion and Size of Autoreactive B-1 Cell Compartment
doi:
Figure Lengend Snippet: Flow cytometric analysis of peritoneal cells of heterozygous or homozygous H+L5, H+L6, H×L, and H3 Tg mice. Cells were isolated from peritoneal cavity of mice at 10–14 wk of age and stained with FITC-anti mouse IgM and Cy-Chrome ® –conjugated anti–mouse B220 (A, E and I); biotin-conjugated anti-Id antibody followed by FITC-conjugated SA and Cy-Chrome ® –conjugated anti-B220 antibody (B and F); biotin-conjugated anti-Id antibody followed by FITC-conjugated SA and PE-conjugated antiallotypic antibody for IgM a (C and G); biotin-conjugated anti-Id antibody followed by FITC-conjugated SA and PE-conjugated anti–mouse Mac-1 (D and H); FITC-conjugated antiallotypic antibody for IgM b (endogenous) and PE-conjugated antiallotypic antibody for IgM a (Tg) (J); and FITC-conjugated goat anti–mouse IgM antibody and PE-conjugated anti–mouse Mac-1 (K). The percentages of the cells of each gated region in total viable cells are indicated. Total peritoneal cell numbers were constant between heterozygous and homozygous Tg mice.
Article Snippet: FITC- or Cy-Chrome ® –conjugated anti–mouse CD45R (B220), FITC- or PE-conjugated anti–mouse IgM a (Igh-6 a ), FITC- or PE-conjugated
Techniques: Isolation, Staining